Safety evaluation of a novel muramidase for feed application (2024)

Lichtenberg, J., Perez Calvo, E., Madsen, K., Østergaard Lund, T., Kramer Birkved, F., van Cauwenberghe, S., Mourier, M., Wulf-Andersen, L., Jansman, A. J. M., & Lopez-Ulibarri, R. (2017). Safety evaluation of a novel muramidase for feed application. Regulatory Toxicology and Pharmacology, 89, 57-69. https://doi.org/10.1016/j.yrtph.2017.07.014

Lichtenberg, J. ; Perez Calvo, E. ; Madsen, K. et al. / Safety evaluation of a novel muramidase for feed application. In: Regulatory Toxicology and Pharmacology. 2017 ; Vol. 89. pp. 57-69.

@article{271287059ee2448f93a3856a375a19b4,

title = "Safety evaluation of a novel muramidase for feed application",

abstract = "Safety evaluation of a muramidase produced by a Trichoderma reesei strain (safe lineage), expressing a muramidase gene isolated from Acremonium alcalophilum is presented. Intended use in feed of this enzyme is as digestive aid in broiler chickens. Muramidase 007, was non-mutagenic and non-clastogenic in vitro, and no adverse effects were observed in 90-day subchronic toxicity studies in rats at doses up to 1132 mg TOS/kg body weight/day. The enzyme did not exhibit, in vitro, skin, nor eye irritation potential. Acute aquatic toxicity evaluated on daphnia and algae showed absence of effect of the enzyme at the standard doses tested. Muramidase 007 was fully tolerated by broiler chickens in a 6-weeks tolerance study showing no adverse effects in any of the dietary treatments (0, 1×, 5× and 10× maximum recommended dose). In conclusion, Muramidase 007 is found to be toxicologically inert, and there are no worker's safety concerns if standard precautions are instituted and a non-dusty formulation is employed. Muramidase 007 is well tolerated by the target species (broiler chickens) and cause no harm to the environment. The beneficial safety evaluation of Muramidase 007 is in line with this type of enzyme that is found ubiquitously in nature.",

keywords = "Chickens, Feed, Feed additive, Lysozyme, Muramidase, Safety, Toxicology, Trichoderma reesei",

author = "J. Lichtenberg and {Perez Calvo}, E. and K. Madsen and {{\O}stergaard Lund}, T. and {Kramer Birkved}, F. and {van Cauwenberghe}, S. and M. Mourier and L. Wulf-Andersen and A.J.M. Jansman and R. Lopez-Ulibarri",

year = "2017",

doi = "10.1016/j.yrtph.2017.07.014",

language = "English",

volume = "89",

pages = "57--69",

journal = "Regulatory Toxicology and Pharmacology",

issn = "0273-2300",

publisher = "Elsevier",

}

Lichtenberg, J, Perez Calvo, E, Madsen, K, Østergaard Lund, T, Kramer Birkved, F, van Cauwenberghe, S, Mourier, M, Wulf-Andersen, L, Jansman, AJM & Lopez-Ulibarri, R 2017, 'Safety evaluation of a novel muramidase for feed application', Regulatory Toxicology and Pharmacology, vol. 89, pp. 57-69. https://doi.org/10.1016/j.yrtph.2017.07.014

Safety evaluation of a novel muramidase for feed application. / Lichtenberg, J.; Perez Calvo, E.; Madsen, K. et al.
In: Regulatory Toxicology and Pharmacology, Vol. 89, 2017, p. 57-69.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Safety evaluation of a novel muramidase for feed application

AU - Lichtenberg, J.

AU - Perez Calvo, E.

AU - Madsen, K.

AU - Østergaard Lund, T.

AU - Kramer Birkved, F.

AU - van Cauwenberghe, S.

AU - Mourier, M.

AU - Wulf-Andersen, L.

AU - Jansman, A.J.M.

AU - Lopez-Ulibarri, R.

PY - 2017

Y1 - 2017

N2 - Safety evaluation of a muramidase produced by a Trichoderma reesei strain (safe lineage), expressing a muramidase gene isolated from Acremonium alcalophilum is presented. Intended use in feed of this enzyme is as digestive aid in broiler chickens. Muramidase 007, was non-mutagenic and non-clastogenic in vitro, and no adverse effects were observed in 90-day subchronic toxicity studies in rats at doses up to 1132 mg TOS/kg body weight/day. The enzyme did not exhibit, in vitro, skin, nor eye irritation potential. Acute aquatic toxicity evaluated on daphnia and algae showed absence of effect of the enzyme at the standard doses tested. Muramidase 007 was fully tolerated by broiler chickens in a 6-weeks tolerance study showing no adverse effects in any of the dietary treatments (0, 1×, 5× and 10× maximum recommended dose). In conclusion, Muramidase 007 is found to be toxicologically inert, and there are no worker's safety concerns if standard precautions are instituted and a non-dusty formulation is employed. Muramidase 007 is well tolerated by the target species (broiler chickens) and cause no harm to the environment. The beneficial safety evaluation of Muramidase 007 is in line with this type of enzyme that is found ubiquitously in nature.

AB - Safety evaluation of a muramidase produced by a Trichoderma reesei strain (safe lineage), expressing a muramidase gene isolated from Acremonium alcalophilum is presented. Intended use in feed of this enzyme is as digestive aid in broiler chickens. Muramidase 007, was non-mutagenic and non-clastogenic in vitro, and no adverse effects were observed in 90-day subchronic toxicity studies in rats at doses up to 1132 mg TOS/kg body weight/day. The enzyme did not exhibit, in vitro, skin, nor eye irritation potential. Acute aquatic toxicity evaluated on daphnia and algae showed absence of effect of the enzyme at the standard doses tested. Muramidase 007 was fully tolerated by broiler chickens in a 6-weeks tolerance study showing no adverse effects in any of the dietary treatments (0, 1×, 5× and 10× maximum recommended dose). In conclusion, Muramidase 007 is found to be toxicologically inert, and there are no worker's safety concerns if standard precautions are instituted and a non-dusty formulation is employed. Muramidase 007 is well tolerated by the target species (broiler chickens) and cause no harm to the environment. The beneficial safety evaluation of Muramidase 007 is in line with this type of enzyme that is found ubiquitously in nature.

KW - Chickens

KW - Feed

KW - Feed additive

KW - Lysozyme

KW - Muramidase

KW - Safety

KW - Toxicology

KW - Trichoderma reesei

U2 - 10.1016/j.yrtph.2017.07.014

DO - 10.1016/j.yrtph.2017.07.014

M3 - Article

AN - SCOPUS:85025083229

SN - 0273-2300

VL - 89

SP - 57

EP - 69

JO - Regulatory Toxicology and Pharmacology

JF - Regulatory Toxicology and Pharmacology

ER -

Lichtenberg J, Perez Calvo E, Madsen K, Østergaard Lund T, Kramer Birkved F, van Cauwenberghe S et al. Safety evaluation of a novel muramidase for feed application. Regulatory Toxicology and Pharmacology. 2017;89:57-69. doi: 10.1016/j.yrtph.2017.07.014